Shyla, B. and Nagendrappa, G. (2013) Redox spectrophotometric method involving electrolytically generated manganese(III) sulphate with diphenylamine for the determination of ascorbic acid present in the samples of various fruits, commercial juices and sprouted food grains. Food Chemistry, 138 (2-3). pp. 2036-2042.
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A spectrophotometric method was developed for ascorbic acid present in various fruits, commercial fruit juices and sprouted food grains. The method involves the oxidation of ascorbic acid with excess manganese(III) following reduction of unreacted manganese(III) with diphenylamine or barium diphenylamine sulphonate forming a product λmax 570 nm the system 1 or 540 nm the system 2 and decrease in the colour intensity is proportional to the concentration of vitamin C with quantification range 0.3-3.0 μg ml -1. The molar absorptivity and Sandell's sensitivity values of the system 1 and the system 2 were 1.829 � 104 and 1.813 � 104 mol-1 cm-1 and 0.0096 and 0.0097 μg cm-2 respectively. The stoichiometry was 4:1 between manganese(III) and diphenylamine. The ascorbic acid contents of the same samples were determined separately following the procedures of the developed method as well as the reference method and the results were comparable.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | article, nonhuman, ascorbic acid, fruit, stoichiometry, sensitivity analysis, chemical reaction, concentration (parameters), oxidation reduction reaction, Oxidation-Reduction, Spectrophotometry, spectrophotometry, Ascorbic Acid, Cereals, Fruit, Reference method, Sulphates, absorption, quantitative analysis, Spectro-photometric method, chemical composition, Sandell's sensitivity, Aniline, Beverages, Diphenylamine, Manganese, Manganese Compounds, manganese sulfate, Sulfates, Sulphonates, Stoichiometry, diphenylamine, chemical analysis, Fruits, Molar absorptivity, Fruit juices, Ascorbic acid, electrolysis, Food grain, fruit juice, Fruits-juices, grain, Manganese(III), Vitamin C |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Arshiya Kousar Library Assistant |
| Date Deposited: | 16 Nov 2019 05:31 |
| Last Modified: | 16 Nov 2019 05:31 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/9601 |
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