Biochemical characterization of protease isoforms in cucumber sap extract

Manjula, B. and Devaraja, S. and Mahadeswaraswamy, Y. H. and Girish, K. S. and Kemparaju, K. (2014) Biochemical characterization of protease isoforms in cucumber sap extract. International Journal of Pharmaceutical and Phytopharmacological Research, 4 (2). pp. 77-83. ISSN 2249-6084

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Abstract

Cucumber (Cucumis sativus. L) fruit is a popular vegetable, which has been widely used in folk medicine as cosmetics, to treat skin-disorders and reduce heat and inflammation. All parts of cucumber fruit are being used for various purposes but the fruit sap although always associated with the fruit pulp, its biochemical and medicinal properties are unexplored. The juicy fluid obtained from the skin peels of cucumber fruit was subjected to acetone precipitation. The precipitate was dissolved in saline to obtain Cucumber Sap Extract (CSE). CSE was assayed for proteolytic activity on several natural protein substrates such as casein, gelatin, hemoglobin, bovine serum albumin, type-I collagen and human fibrinogen. Biochemical properties such as effect of pH, temperature, inhibitors, metal ions, organic solvents and detergents on the proteolytic activity were studied. Medicinal properties such as effect on the formation of plasma clot, dissolution of clot and degradation of collagen type I was determined. Activity staining on casein substrate gel revealed several translucent activity bands that are distributed in the wide molecular mass range indicating several isoforms and further their differential sensitivity and stability towards pH, temperature, protease inhibitors, metal ions, organic solvents and detergents. CSE was devoid of toxic properties such as edema and hemorrhage. In this preliminary study an attempt is made to show the presence of variety of proteases isoforms for commercial exploration in food, drug, cosmetic, leather and detergent industries.

Item Type:	Article
Uncontrolled Keywords:	Cucumber Sap Extract and Cucumber Protease and Collagen Degradation and Clot Dissolution and Fibrinogen Degradation and Plant protease.
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Users 19 not found.
Date Deposited:	04 Oct 2019 09:23
Last Modified:	04 Oct 2019 09:23
URI:	http://eprints.uni-mysore.ac.in/id/eprint/8707

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