Shashidharamurthy, R. and Kemparaju, K. (2006) A neurotoxic phospholipase A2 variant: Isolation and characterization from eastern regional Indian cobra (Naja naja) venom. Toxicon, 47 (7). 727 - 733. ISSN 1879-3150
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CM-Sephadex C-25 column chromatography profile of Indian cobra (Naja naja) venom from eastern region showed a distinct and a dominant phospholipase peak, peak-10, while it was not seen in either southern or western venom samples. Peak-10 was subjected to CM-Sephadex C-25 and Sephadex G-50 column chromatography to isolate NN-X-PLA2. NN-X-PLA2 is a single chain protein with the relative molecular weight of 10kDa by SDS-PAGE. It was toxic to mice with an LD50 value 0.098mg/kg body weight (i.p.) and the mice exhibited acute neurotoxic symptoms. Upon indirect stimulation, it inhibited the twitching of frog's gastrocnemius muscle in a dose dependent manner. NN-X-PLA2 was weakly anticoagulant and devoid of cytotoxicity, myotoxicity, hemorrhage, edema inducing, and directlytic activities and effects on platelet aggregation process. Upon chemical modification independently with p-bromophenacyl bromide and acetic anhydride, NN-X-PLA2 lost both enzymatic and toxic properties.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Neurotoxic PLA, venom, Venom variability, PLA variant |
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | LA manjunath user |
| Date Deposited: | 19 Aug 2019 11:04 |
| Last Modified: | 19 Aug 2019 11:04 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/6778 |
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