Satish, S. and Tejaswini, J. and Krishnakantha, T. and Veerabasappa Gowda, T. (2004) Purification of a class B1 platelet aggregation inhibitor phospholipase A(2) from Indian cobra (Naja naja) venom. BIOCHIMIE, 86 (3). pp. 203-210. ISSN 0300-9084
Full text not available from this repository. (Request a copy)Abstract
A platelet aggregation inhibitor phospholipase A, (NND-IV-PLA(2)) was isolated from Naja naja (Eastern India) venom by a combination of cation and anion exchange chromatography. NND-IV-PLA(2) is the most catalytically active enzyme isolated from the Indian cobra venom. The acidic PLA(2) profile of Eastern regional Indian cobra venom is distinctly different from that of the western regional venom. However the acidic PLA(2)s from both the regions follow the pattern of increasing catalytic activity with increase in acidic nature of the PLA(2) isoform. NND-IV-PLA(2) is a Class B1 platelet aggregation inhibitor and inhibits platelet aggregation induced by ADP, collagen and epinephrine. Modification of active site histidine abolishes both catalytic activity and platelet aggregation inhibition activities while aristolochic acid, a phospholipase A(2) inhibitor has only partial effect on the two activities. (C) 2004 Elsevier SAS. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Class B1 platelet aggregation inhibitor; phospholipase A(2); aristolochic acid; Naja naja |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 23 Aug 2019 13:50 |
| Last Modified: | 12 Jul 2022 07:57 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/6608 |
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