Chiral separation of carboprost isomers by normal phase LC using amylose chiral stationary phase

Hiriyanna, S. G. and Basavaiah, K. and Dhayanithi, V. and Pati, H. N. (2008) Chiral separation of carboprost isomers by normal phase LC using amylose chiral stationary phase. Chromatographia, 68 (7-8). pp. 501-505. ISSN 1612-1112

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Abstract

LC separation of carboprost diastereomers in bulk drug was developed and validated using normal-phase amylose stationary phase Chiralpak AD-H. The effect of the organic modifiers, namely 2-propanol and ethanol in the mobile phase was optimized in order to obtain the best separation. The retention time of (R)-carboprost and (S)-carboprost were 15.3 and 17.1 min, respectively. Calibration curves were linear over the range of 0.2–1.0%, with the regression coefficient (R 2) of 0.9997. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.07 and 0.2%, respectively. The method was accurate, precise and suitable to use for the purpose of controlling unwanted (R)-isomer in the carboprost active pharmaceutical ingredient. This method can be successfully applied to the analysis of chiral purity of carboprost in pharmaceutical bulk drug samples.

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Manjula P Library Assistant
Date Deposited:	16 Aug 2019 11:31
Last Modified:	16 Aug 2019 11:31
URI:	http://eprints.uni-mysore.ac.in/id/eprint/6448

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