Ponnappa, K. C. and Pushpa Saviour and Ramachandra, N. B. and Manjunatha Kini, R. and Veerabasappa Gowda, T. (2008) INN-toxin, a highly lethal peptide from the venom of Indian cobra (Naja naja) venom—Isolation, characterization and pharmacological actions. Peptides, 29 (11). 1893 - 1900. ISSN 1873-5169
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A novel toxic polypeptide, INN-toxin, is purified from the venom of Naja naja using combination of gel-permeation and ion-exchange chromatography. It has a molecular mass of 6951.6Da as determined by MALDI-TOF/MS and the N-terminal sequence of LKXNKLVPLF. It showed both neurotoxic as well as cytotoxic activities. INN-toxin is lethal to mice with a LD50 of 1.2mg/kg body weight. IgY raised in chicks against basic peptide pool neutralized the toxicity of INN-toxin. INN-toxin did not inhibit cholinesterase activity. It is toxic to Ehrlich ascites tumor (EAT) cells, but it is not toxic to leukocyte culture. The toxin appears to be specific in its mode of action. Interaction of N-bromosuccinamide (NBS) with the peptide resulted in the modification of tryptophan residues and loss of lethal toxicity of INN-toxin.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Postsynaptic neurotoxins, Cytotoxins, Ehrlich ascites tumor cells, Leukocytes, IgY, -bromosuccinamide, |
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Manjula P Library Assistant |
| Date Deposited: | 16 Aug 2019 09:44 |
| Last Modified: | 16 Aug 2019 09:44 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/6439 |
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