Spectrophotometric determination of isoxsuprine hydrochloride as base form in pharmaceutical formulation through charge transfer complexation

Kalsang, Tharpa and Vinay, K. B. (2010) Spectrophotometric determination of isoxsuprine hydrochloride as base form in pharmaceutical formulation through charge transfer complexation. Croatica Chemica Acta, 83 (4). pp. 415-420. ISSN 1334-417X

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Abstract

Two simple and selective spectrophotometric methods are described for the determination of isoxsuprine hydrochloride (ISX·HCl) as base form in bulk drug and in injections and tablets. The methods are based on the molecular charge-transfer complexation of ISX base (ISX) with either p-chloranilic acid (PCA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). The intense colored radical anions formed on dissociation, are quantitated at 520 nm (PCA method) or 600 nm (DDQ method). The optimum assay conditions were optimized. Beer's law is obeyed in the concentration ranges 5.28 × 10–5 to 42.2 × 10–5 mol dm–3 in PCA method and 0.79 × 10–5 to 10.6 × 10–5 mol dm–3 in DDQ method, with respective molar absorptivity values of 1.32 × 103 and 6.55 × 103 dm3 mol–1 cm–1. The reaction stoichiometry in both methods was evaluated by either Job’s method or limiting logarithmic method and was found to be 1:1 (ISX: PCA, ISX: DDQ). Developed methods were validated according to ICH guidelines and found to be accurate and precise

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	LA manjunath user
Date Deposited:	29 Jul 2019 09:52
Last Modified:	25 Oct 2019 11:00
URI:	http://eprints.uni-mysore.ac.in/id/eprint/6181

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