Seema, M. and Punith, B. D. and Devaki, N. S. (2012) A simple and rapid nuclear staining method for Rhizoctonia solani Kuhn. Biotechnic & Histochemistry, 87 (3). pp. 169-171. ISSN 1473-7760
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Official URL: https://doi.org/10.3109/10520295.2011.577097
Abstract
AbstractWe developed a modified staining technique using acridine orange to stain the nuclei of Rhizoctonia solani. Acridine orange solution was prepared in acetic acid buffer, pH 7.2. Staining for 15 min was critical for observing the nuclei. All of the isolates were found to be multinucleated. The nuclei appeared bright green with light orange background. This method is simple, rapid and reproducible.
| Item Type: | Article |
|---|---|
| Subjects: | B Life Science > Molecular Biology |
| Divisions: | Yuvaraj college > Molecular biology |
| Depositing User: | C Swapna Library Assistant |
| Date Deposited: | 22 Jul 2019 10:26 |
| Last Modified: | 22 Jul 2019 10:26 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/5440 |
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