Determination of ethionamide in pharmaceutical by spectrophotometry through oxidation reaction with potassium iodate

Qarah, Nagib A. S. and Basavaiah, K. (2017) Determination of ethionamide in pharmaceutical by spectrophotometry through oxidation reaction with potassium iodate. Thai Journal of Pharmaceutical Sciences, 41 (4). pp. 150-156.

Text (Full Text) Determination of ethionamide in pharmaceutical by spectrophotometry through oxidation reaction with potassium iodate.pdf - Published Version Restricted to Registered users only Download (601kB) | Request a copy

Abstract

Objective: To described two new spectrophotometric methods for the determination of ethionamide (ETM) in pure and tablet forms. Methods: The developed first method is based on the oxidation of ETM by KIO3 in H2SO4 medium, and the in situ generated iodine is extracted into chloroform and measured at 520 nm, While in the second method, the same iodine is complexed with starch and the intense blue colored complex is measured at 580 nm. Different variables affecting the reactions were carefully studied and optimized. Results: Beer’s law is obeyed in the concentration ranges of 20–400 and 5–125 μg/mL with Methods A and B, respectively, with the respective molar absorptivity (ε) values of 4.04 × 102 and 1.39 × 103 L/mol/cm. The Sandell sensitivity values were calculated to be 0.4113 and 0.1193 μg/mL. The limits detection and quantification were calculated to be 0.85 and 2.56 μg/mL for Method A and 0.27 and 0.81 μg/mL for Method B. The intraday and interday precisions expressed as relative standard deviation (%RSD) were found to be in the range 1.46–2.32%. The accuracy expressed as relative error (%RE) was better than 2.35%. The methods were also found to be robust and rugged as found from low values of RSD. Conclusion: The two developed methods were applied to the determination of ETM in tablets and no interference was observed from common tablet excipients.

Item Type:	Article
Uncontrolled Keywords:	Determination, ethionamide, pharmaceuticals, potassium iodate, spectrophotometry
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	09 Jul 2019 06:19
Last Modified:	09 Jul 2019 06:19
URI:	http://eprints.uni-mysore.ac.in/id/eprint/4978

Actions (login required)

View Item