Soumya, K. and Narasimha Murthy, K. and Sreelatha, G. L. and Tirumale, S. (2018) Characterization of a red pigment from Fusarium chlamydosporum exhibiting selective cytotoxicity against human breast cancer MCF‐7 cell lines. Journal of Applied Microbiology, 125 (1). pp. 148-158. ISSN 1365-2672
Full text not available from this repository. (Request a copy)Abstract
Aim This research aims to characterize the pigment produced by isolated fungi and to evaluate its anticancer activities. Methods and Results Pigment‐producing fungi was isolated and identified as Fusarium chlamydosporum. The pigment was extracted with chloroform, purified by preparative TLC and characterized by Fourier transmittance infrared, electron spray ionization mass spectroscopy, liquid chromatography mass spectroscopy and nuclear magnetic resonance (NMR) (1HNMR, 13C NMR) spectral analysis, which revealed the pigment to be ‘long chain hydrocarbons with poly unsaturated groups’ (m/z 702). Pigment stability varied with different pH, temperature and sunlight conditions. The pigment‐induced cell death in human breast adenocarcinoma cells MCF‐7 and showed no significant toxicity in CHOK 1 cells. Lipid peroxidation assay revealed that treatment with pigment was able to reduce the lipid peroxidation caused by H2O2 in MCF‐7 cells. Conclusions The F. chlamydosporum pigment is a compound of long‐chain hydrocarbons with poly unsaturated groups, possessing selective cytotoxicity in MCF‐7 cancer cell lines. Significance and Impact of the Study The pigment can be used as a colouring agent in cosmetics. Its anticancer potential can be used in production of therapeutics in increasing demand cancer research.
| Item Type: | Article |
|---|---|
| Divisions: | Department of > Biotechnology |
| Depositing User: | Manjula P Library Assistant |
| Date Deposited: | 08 Jul 2019 10:20 |
| Last Modified: | 08 Jul 2019 10:20 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/4902 |
Actions (login required)
 |
View Item |
