Nagaraja, P. and Avinash, K. and Shivakumar, A. and Dinesh, R. and Shrestha, Ashwinee Kumar (2010) Simple and sensitive method for the quantification of total bilirubin in human serum using 3-methyl-2-benzothiazolinone hydrazone hydrochloride as a chromogenic probe. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 77 (4). 782 - 786. ISSN 1386-1425
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We here describe a new spectrophotometric method for measuring total bilirubin in serum. The method is based on the cleavage of bilirubin giving formaldehyde which further reacts with diazotized 3-methyl-2-benzothiazolinone hydrazone hydrochloride giving blue colored solution with maximum absorbance at 630nm. Sensitivity of the developed method was compared with Jendrassik-Grof assay procedure and its applicability has been tested with human serum samples. Good correlation was attained between both methods giving slope of 0.994, intercept 0.015, and R2=0.997. Beers law obeyed in the range of 0.068–17.2μM with good linearity, absorbance y=0.044Cbil+0.003. Relative standard deviation was 0.006872, within day precision ranged 0.3–1.2 and day-to-day precision ranged 1–6. Recovery of the method varied from 97 to 102. The proposed method has higher sensitivity with less interference. The obtained product was extracted and was spectrally characterized for structural confirmation with FT-IR, 1H NMR.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bilirubin, Spectrophotometry, MBTH, Bilirubin in serum, Human serum, Jendrassik-Grof |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | LA manjunath user |
| Date Deposited: | 05 Jul 2019 10:28 |
| Last Modified: | 30 Jul 2019 06:14 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/4778 |
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