Bioactive potential of endophytic Myrothecium sp. isolate M1-CA-102, associated with Calophyllum apetalum

Ruma, K. and Sunil, K. and Prakash, H. S. (2014) Bioactive potential of endophytic Myrothecium sp. isolate M1-CA-102, associated with Calophyllum apetalum. Pharmaceutical Biology, 52 (6). pp. 665-676. ISSN 1744-5116

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Abstract

Context: Endophytes colonizing medicinal plants are diverse, constituting a rich bioresource for novel natural products. Objective: Myrothecium sp. isolate M1-CA-102 was the most promising among the 16 Myrothecium isolates screened. The bioactive potential of the crude extract from the Calophyllum apetalum Willd. endophytic Myrothecium sp. (Alb. & Schwein.) Ditmar (Incertae sedis) isolate M1-CA-102 and its thin layer chromatography (TLC) fractions were screened based on antioxidant, anti-inflammatory, antimicrobial activities, and cytotoxicity. Materials and methods: The antioxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2�-azinobis-(3- ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging capacities. Further, 15-lipoxygenase (15-LOX) and human cyclooxygenase-2 (COX-2) inhibition were assessed at different concentrations (25, 50, and 100μg/mL for the crude extract, 5, 25, and 50μg/mL for the TLC fractions). DNA-nicking assay as an indicator of the capacity of extracts to scavenge hydroxyl radical was recorded at a concentration of 50μg/mL. Cell cytotoxicity was recorded by colorimetric 3-(4,5-dimethylthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Antibacterial (Bacillus subtilis) and anti-Candida (Candida albicans) assays were performed by the microdilution method. Results: The DPPH and ABTS IC50 values of M1-CA-102 extract were 10 and 6μg/mL compared with 6.1 and 7.03μg/mL for the positive control quercetin. The cytotoxicity IC50 value of M1-CA-102 extract was 37μg/mL, while the M-I TLC fraction was 21μg/mL. The M1-CA-102 extract gave an IC50 value of 58 and 8μg/mL for 15-LOX and COX-2, respectively. The MIC values for antimicrobial activity for M1-CA-102 extract ranged from 35 to 54μg/mL, while for the TLC fractions, it ranged from 91 to 515μg/mL. Conclusion: The results indicate that Myrothecium M1-CA-102 isolated from C. apetalum is a potential source of natural metabolites of pharmaceutical importance. © 2014 Informa Healthcare USA, Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	controlled study, human, nonhuman, drug screening, Humans, dose response, Dose-Response Relationship, Drug, antibacterial activity, antifungal activity, Bacillus subtilis, Candida albicans, procedures, antiinflammatory activity, antioxidant, antioxidant activity, Antioxidants, biological product, cyclooxygenase 2, cyclooxygenase 2 inhibitor, Cyclooxygenase 2 Inhibitors, 1, plant extract, article, fungal extract, minimum inhibitory concentration, 2, thin layer chromatography, isolation and purification, Plant Extracts, microbial sensitivity test, Microbial Sensitivity Tests, drug purification, biological activity, drug isolation, endophyte, Endophytes, HeLa cell line, Biological Products, 1 diphenyl 2 picrylhydrazyl, IC 50, arachidonate 15 lipoxygenase, quercetin, 3 (4, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide, 2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), HeLa Cells, cytotoxicity test, Calophyllum, Calophyllum apetalum, Myrothecium
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	23 Aug 2019 05:18
Last Modified:	23 Aug 2019 05:18
URI:	http://eprints.uni-mysore.ac.in/id/eprint/4394

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