Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein

Zarena, A. S. and Shubha Gopal and Vineeth, R. (2014) Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein. Journal of Analytical Methods in Chemistry, 2014.

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Abstract

In the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is �29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33 M, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44 M in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity against Pseudomonas aeruginosa (20 ± 3.64 mm) and Staphylococcus aureus (19 ± 1.53 mm) at 200 g/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL.

Item Type:	Article
Uncontrolled Keywords:	drug cytotoxicity, human, human cell, unclassified drug, lymphocyte, antibacterial activity, antiinfective agent, bacterial strain, priority journal, Staphylococcus aureus, antioxidant, antioxidant activity, protein analysis, molecular weight, article, high performance liquid chromatography, lipid peroxidation, cell death, Pseudomonas aeruginosa, polyacrylamide gel electrophoresis, DNA damage, cell protection, vegetable protein, agathi leaf protein, erythrocyte ghost, linolenic acid
Subjects:	B Life Science > Microbiology
Divisions:	Department of > Microbiology
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	31 Aug 2019 05:05
Last Modified:	31 Aug 2019 05:06
URI:	http://eprints.uni-mysore.ac.in/id/eprint/4323

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