Inhibitory effect of banana (Musa sp. var. Nanjangud rasa bale) flower extract and its constituents Umbelliferone and Lupeol on α-glucosidase, aldose reductase and glycation at multiple stages

Ramu, R. and Shirahatti, P. S. and Zameer, F. and Lakshmi Ranganatha, V. and Nagendra Prasad, M. N. (2014) Inhibitory effect of banana (Musa sp. var. Nanjangud rasa bale) flower extract and its constituents Umbelliferone and Lupeol on α-glucosidase, aldose reductase and glycation at multiple stages. South African Journal of Botany, 95. pp. 54-63. ISSN 0254-6299

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Official URL: https://doi.org/10.1016/j.sajb.2014.08.001

Abstract

Postprandial hyperglycaemia is characterized as the earliest symptom of diabetes and its management attenuates several of the associated secondary complications. In this context, we investigated the role of ethanol extract of banana flower (EF) for its antihyperglycaemic effects. The EF showed a strong inhibition towards α-glucosidase and pancreatic amylase which play a vital role in clinical management of postprandial hyperglycaemia. The major active compounds present in EF were identified as Umbelliferone (C1) and Lupeol (C2) using various spectroscopic methods. C1 (IC50: 7.08±0.17μg/ml) and C2 (IC50: 7.18±0.14μg/ml) were found to inhibit α-glucosidase in a non-competitive mode of inhibition, with low Ki values. Further, in vitro glycation assays showed that EF and its compounds prevented each stage of protein glycation and formation of its intermediary compounds. EF, C1 and C2 also exhibited a potent inhibition on aldose reductase with IC50 values of 2.25±0.29, 1.32±0.22 & 1.53±0.29μg/ml respectively. Our results suggest that, the observed potential of EF in antihyperglycaemic activity via inhibition of α-glucosidase and in antidiabetogenic effect by inhibition of polyol pathway and protein glycation is more likely to be attributed to the presence of C1 and C2.

Item Type: Article
Uncontrolled Keywords: enzyme activity, protein, fruit, disease control, ethanol, Musa sp.
Subjects: C Chemical Science > Chemistry
Divisions: Yuvaraj college > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 31 Aug 2019 10:36
Last Modified: 31 Aug 2019 10:36
URI: http://eprints.uni-mysore.ac.in/id/eprint/4286

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