Quantification of antioxidants by using chlorpromazine hydrochloride: Application of the method to food and medicinal plant samples

Nagaraja, P. and Aradhana, N. and Suma, A. and Shivakumar, A. and Chamaraja, N. A. (2014) Quantification of antioxidants by using chlorpromazine hydrochloride: Application of the method to food and medicinal plant samples. Analytical Sciences, 30 (2). pp. 251-256. ISSN 0910-6340

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Official URL: https://doi.org/10.2116/analsci.30.251

Abstract

Chlorpromazine hydrochloride (CPH) (3-(2-chloro-phenothiazine-10-yl)-propyl] dimethylamine hydrochloride) has been the subject of a large number of studies employing a broad spectrum of oxidants, and chosen to examine the course of electron transfer reactions. We report on a method to determine the antioxidant activity of some food and medicinal plants using the oxidation of CPH by chromium(VI) to form a stable CPH radical in the 1:1 orthophosphoric acid-ethyl alcohol (OPA-EtOH) medium. The pink color of the control solution was measured at λ max of 530 nm. Nine standard antioxidants have been studied by this method, along with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The EC 50 , TEC 50 , antioxidant efficacy and the stoichiometric values for antioxidants have been evaluated. The radical scavenging activity expressed as EC 50 ranged from 9.2 μg/mL in Camellia sinensis to 448.18 μg/mL in Cuminum cyminum. The application of a simple and versatile antioxidant capacity assay for dietary polyphenols and medicinal plant extracts, which are commonly used in Ayurveda opens its relevance in the field of antioxidant analysis.

Item Type: Article
Uncontrolled Keywords: chemistry, antioxidant, Antioxidants, article, medicinal plant, isolation and purification, free radical, oxidation reduction reaction, Oxidation-Reduction, methodology, Plants, Free Radicals, Medicinal, food analysis, Food Analysis, solvent, Solvents, chlorpromazine, Chlorpromazine
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 29 Jun 2019 06:04
Last Modified: 29 Jun 2019 06:04
URI: http://eprints.uni-mysore.ac.in/id/eprint/3970

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