A gradient based facile HPLC method for simultaneous estimation of antioxidants extracted from tea powder

Nanjegowda, S. H. and Manasa G. Papanna and Achar, Raghu Ram and Rangappa, K. S. and Mallu, Puttaswamappa and Nanjunda Swamy, S. (2016) A gradient based facile HPLC method for simultaneous estimation of antioxidants extracted from tea powder. Journal of Food Science and Technology, 53 (5). pp. 2253-2259. ISSN 0975-8402

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Abstract

A new simple, rapid and precise RP-HPLC method was developed for the extraction and quantitative estimation of caffeine (C), (−)-epigallocatechin gallate (EGCG), (+)-catechin(Ct), (−)-epicatechin(EC), and (−)-epicatechin gallate (ECG) (collectively named as Tea Powder Bioactives TPBAs) extracted from tea powder using different ratios of ethanol: water. The simultaneous determination of TPBAs was performed using the UV spectrophotometric method which employs the absorbance at 205 nm (λmax of caffeine and polyphenols). This method is a gradient based HPLC method with a flow rate of 0.8 mL/min using Inertsil ODS 100 × 4.6 mm, 3 μm column with methanol and ammonium dihydrogen phosphate (pH-2.8) as mobile phase. The method was validated in terms of specificity, precision, linearity, accuracy, limit of quantification (LOQ), and limit of detection (LOD). The linearity of the proposed method was investigated for concentration ranging between 0.5–60 μg/mL with regression co-efficient, R2 = 0.999–1.0. This method estimates all the TPBAs simultaneously with enhanced precision and linearity as per the ICH guidelines. Also, to confirm the individual TPBA, the antioxidant property of the each TPBA was analyzed which was commensurate with that of the previous reports.

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry Department of > Institution of Excellence
Depositing User:	Manjula P Library Assistant
Date Deposited:	21 Jun 2019 08:16
Last Modified:	05 Sep 2023 09:22
URI:	http://eprints.uni-mysore.ac.in/id/eprint/3585

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