Roopa, R. A. and Mantelingu, K. and Mridula Guin (2017) Rapid and selective spectrophotometric assay for micromolar level determination of hydrogen peroxide based on biocatalysis of horseradish peroxidase. Analytical Chemistry Letters, 7 (6). pp. 779-791.
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AbstractHydrogen peroxide (H2O2) is an essential compound in food, pharmaceutical, clinical, industrial and environmental analysis. H2O2 has been widely used for preservation of raw milk due to its bactericidal properties. However, excess of H2O2 can be deleterious on the nutritional value of milk due to degradation of folic acid. A simple and sensitive spectrophotometric assay was developed for the quantification of H2O2 in milk. This method is based on coupling of Iso-nicotinic acid hydrazide (INH) and Resorcinol (RC) as chromogenic co-substrates in presence of horseradish peroxidase (HRP) and H2O2 to form a red colored product, which has strong absorbance at λmax = 520 nm. The effects of different parameters on the sensitivity of the proposed assay were studied and optimized. Under optimized condition a linearity response for H2O2 are 0.5 μM - 64 μM and 0.5 μM -128 μM for kinetic and fixed time methods, respectively. The detection limit, quantification limit and molar extension co-efficient values for H2O2 assay were found to be 0.87 μM, 2.63 μM and 0.328×105 L/mol/cm, respectively. The Michaelis-Menten (Km) constant and catalytic efficiency were 36.27 μM and 2.0927 ×105 M-1 min-1. The proposed method produced recovery in the range of 99-102.8 % for milk samples and 98.2-101.8 % for rain water samples. Some of the important advantages of the proposed method are, use of inexpensive reagents, simple operation conditions, rapid analysis, wide linear range, high sensitivity, free from interfering substances and precision comparable to the reference methods.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Horseradish peroxidase, hydrogen peroxide assay, milk sample, rain water sample, iso-nicotinicacidhydrazide, resorcinol |
| Subjects: | C Chemical Science > Chemistry |
| Depositing User: | C Swapna Library Assistant |
| Date Deposited: | 19 Jun 2019 04:58 |
| Last Modified: | 28 Jun 2019 09:09 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/3376 |
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