Evidence for peroxidase activity in Caralluma umbellata

Achar, R. R. and Venkatesh, B. K. and Sharanappa, P. and Priya, B. S. and Swamy, S. N. (2014) Evidence for peroxidase activity in Caralluma umbellata. Applied Biochemistry and Biotechnology, 173 (8). pp. 1955-1962. ISSN 1559-0291

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Abstract

Vast applications of peroxidases create an increasing demand to characterize peroxidases from new sources with more applicability potential. The aim of the present study was to check the presence of peroxidase activity from Caralluma umbellata. This is the first report on the C. umbellata peroxidase (CUP). The presence of peroxidase was revealed by the histochemical analysis of the stem sections, zymographic studies, and in vitro peroxidase activity assay using various reducing substrates viz., 2, 2�-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), guaiacol, o-dianisidine, and ferulic acid. The band pattern in zymogram confirms that CUP has a molecular weight less than that of horseradish peroxidase (44kDa). Comparative evaluation of peroxidase activity of CUP with respect to horseradish peroxidase (HRP) indicates that CUP catalyzes ABTS and ferulic acid in a similar pattern as HRP but with guaiacol, the extent of catalysis shown by CUP over HRP is high. The standard inhibitors sodium azide and sodium meta bisulphite inhibited CUP activity in a dose dependent manner. © 2014 Springer Science+Business Media.

Item Type:	Article
Uncontrolled Keywords:	controlled study, in vitro study, nonhuman, unclassified drug, chemistry, metabolism, genetics, Plant Proteins, enzyme activity, peroxidase, plant, molecular weight, enzymology, enzyme assay, enzyme inhibition, plant extract, article, enzyme purification, catalysis, 2, Apocynaceae, plant cell, drug activity, zymography, Biocatalysis, Peroxidases, biocatalysis, cell wall, kinetics, Kinetics, 2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), Sodium, vegetable protein, enzyme specificity, Substrate Specificity, coumaric acid, Coumaric Acids, Histochemical analysis, histochemistry, Alcohols, Armoracia rusticana, Asclepiadaceae, Caralluma umbellata, Caralluma umbellata peroxidase, Caralluma umbellata Peroxidase, Comparative evaluations, CUP, dianisidine, Dose-dependent manner, ferulic acid, guaiacol, Guaiacol, horseradish peroxidase, Horseradish peroxidase, L-AAO, Peroxidase activities, sodium azide, sodium metabisulfite
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	11 Jun 2019 06:16
Last Modified:	08 Jul 2022 06:42
URI:	http://eprints.uni-mysore.ac.in/id/eprint/2924

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