Use of alizarin red S as a chromogenic agent for the colorimetric determination of dothiepin hydrochloride in pharmaceutical formulations

Abdulrahman, S. A. M. and Basavaiah, K. (2014) Use of alizarin red S as a chromogenic agent for the colorimetric determination of dothiepin hydrochloride in pharmaceutical formulations. Journal of Saudi Chemical Society, 18 (2). pp. 107-114. ISSN 1319-6103

[img] Text (Full Text)
Che_2014_Basavaiah.pdf - Published Version
Restricted to Registered users only
Download (687kB) | Request a copy
Official URL: https://doi.org/10.1016/j.jscs.2011.05.018

Abstract

The present study describes two simple, rapid, selective and cost-effective spectrophotometric methods for the determination of dothiepin hydrochloride (DOTH), an antidepressant drug, in bulk drug and pharmaceutical formulations. The first method (method A) is based on the formation of yellow colored ion-pair complex between DOTH and alizarin red S (ARS) in acid medium which was extracted into dichloromethane and the absorbance was measured at 445nm. The second method (method B) is based on the breaking of the yellow DOTH-ARS ion-pair complex in alkaline medium followed by the measurement of the violet color free dye at 570nm. Under the optimized conditions, Beer's law is obeyed over the concentration ranges of 2.50-55.0 and 1.00-35.0μgml-1 DOTH for method A and method B, respectively. The molar absorptivity, Sandell's sensitivity, detection and quantification limits are also calculated. The methods were validated for intra-day and inter-day accuracy and precision; selectivity and robustness and ruggedness. The proposed methods were applied successfully to the determination of DOTH in pure drug and commercial formulations. The accuracy and reliability of the proposed methods were further established by parallel determination by the official method and also by recovery studies via standard addition technique.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 13 Jun 2019 10:33
Last Modified: 22 Jun 2022 09:20
URI: http://eprints.uni-mysore.ac.in/id/eprint/2909

Actions (login required)

View Item View Item
uomeprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.