Evaluation of substrates for mass production of trichoderma harzianum and its compatibility with chlorpyrifos + cypermethrin

Siddhartha, N. S. and Amara, K. V. and Ramya Mol, K. A. and Saju, K. A. and Harsha, K. N. and Sharanappa, P. and Pradip Kumar, K. (2017) Evaluation of substrates for mass production of trichoderma harzianum and its compatibility with chlorpyrifos + cypermethrin. International journal of current microbiology and applied sciences, 6 (8). pp. 3628-3635. ISSN 2319-7706

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Abstract

Coir pith compost, rice husk and sugarcane bagasse with and without 5% Jaggery solution as nutritional supplement was evaluated for the mass production of Trichoderma harzianum in comparison with coffee husk (parchment). On incubation up to 60 days, maximum number of propagules were recorded in coir pith compost (43 × 1010), coir pith compost + 5% jaggery (32.33 × 1010) and sugarcane bagasse (21 × 1010) followed by rice husk (38 × 1010) and rice husk + 5% jaggery (23.33 × 1010). Coir pith compost and sugarcane bagasse recorded more population than other substrates and hence could be used in mass production. In another experiment, the compatibility of T. harzianum and insecticide combination, Chlorpyrifos 50% + Cypermethrin 5% was studied by poisoned food technique on PDA medium at concentrations of 55, 83, 110, 138, 165, 193 and 220 ppm. In the first set, the insecticide was added to the autoclaved medium (hence not heated) and in the second set the insecticide was autoclaved along with the medium. In both the cases, the colony growth was very slow initially for 5 days, however continued to grow as evident from the measurements on 10th and 15th day. Thus the insecticide was partially compatible with T. harzianum in in-vitro studies at all the concentration levels. Later, T. harzianum and Chlorpyrifos + Cypermethrin were incorporated into soil taken in containers so as to get the above insecticide concentrations and studied the compatibility by monitoring the population by serial dilution plate technique. After 30 days, it was found that the propagules in all the insecticide concentrations were on par with each other. Hence it is implied that the insecticide did not affect the population of T. harzianum in in-vivo conditions and found compatible for field application.

Item Type:	Article
Uncontrolled Keywords:	Chlorpyrifos, compatibility, Cypermethrin,Mass production, Substrates and Trichoderma
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	C Swapna Library Assistant
Date Deposited:	06 Jun 2019 10:16
Last Modified:	06 Jun 2019 10:16
URI:	http://eprints.uni-mysore.ac.in/id/eprint/2684

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