Sensitive liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole sodium in human urine

Bhaskara, B. L. and Anil Kumar, U. R. and Basavaiah, K. (2011) Sensitive liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole sodium in human urine. Arabian Journal of Chemistry, 4 (2). pp. 163-168. ISSN 1878-5352

Text (Full Text) Che_2011_Anil Kumar _ Basavaiah.pdf - Published Version Download (821kB)

Abstract

A sensitive and selective liquid chromatographic tandem mass spectrometric (LC-MS-MS) method was developed to determine pantoprazole sodium (PNT) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C-18 column (symmetry 3.5 mu m; 75 mm x 4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (90:10, v/v). The method was linear over a concentration range of 1-100 ng mL(-1). The lower limit of quantitation was 1 ng mL(-1). The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was < 10.5%. The accuracy determined at three concentrations (8.0, 50.0 and 85.0 ng mL(-1) PNT) was within +/- 1.25% in terms of relative errors. (c) 2010 King Saud University. Production and hosting by Elsevier B.V. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	Pantoprazole sodium; Quantification; LC-MS
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Users 23 not found.
Date Deposited:	04 Jul 2019 09:41
Last Modified:	03 Jun 2023 11:22
URI:	http://eprints.uni-mysore.ac.in/id/eprint/2528

Actions (login required)

View Item