Prevalence of fumonisin producing fusarium verticillioides associated with cereals grown in Karnataka (India)

Deepa, N. and Nagaraja, H. and Sreenivasa, M. Y. (2016) Prevalence of fumonisin producing fusarium verticillioides associated with cereals grown in Karnataka (India). Food Science and Human Wellness, 5 (3). 156 - 162.

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Official URL: https://doi.org/10.1016/j.fshw.2016.07.001

Abstract

A total of 135 cereal samples were collected from different districts of Karnataka state, India in which 69 samples were infected with Fusarium species. Among these 51 samples were having Fusarium verticillioides infection and among them 42 samples were positive for fumonisin production. Per cent incidence and frequency were high in maize samples with 33.12 and 47.54, respectively followed by paddy and sorghum, while pearl millet was free from F. verticillioides infection. Relative density of F. verticillioides association was 59.50 among the screened samples. A total of 326 Fusarium species were isolated by screening 135 cereal samples and among these 194 isolates of F. verticillioides scored positive for VERTF-1 and VERTR species-specific pair of primers. Further amplification with VERTF-1 and VERTF-2 pair of primers recorded 176 isolates of fumonisin producing F. verticillioides. The study revealed high incidence, frequency and relative density of fumonisin producing F. verticillioides and production of fumonisins in cereals. It was amplified using one forward and two reverse primers to discriminate fumonisin producing from fumonisin non-producing F. verticillioides which stresses the need for the development of managemental stratergies before they enter into the food chain.

Item Type: Article
Uncontrolled Keywords: Fumonisin, , IGS, Cereal, Maize, Paddy, Sorghum
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Manjula P Library Assistant
Date Deposited: 01 Jun 2019 05:36
Last Modified: 18 Sep 2019 11:20
URI: http://eprints.uni-mysore.ac.in/id/eprint/2002

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