GC-MS profiling and efficacy of Crotalaria ramosissima Roxb. leaf extracts in controlling termite, Odontotermes obesus

Lanchana, H. A. and Basavarajappa, S. and Rajkumar, H. G. (2024) GC-MS profiling and efficacy of Crotalaria ramosissima Roxb. leaf extracts in controlling termite, Odontotermes obesus. Journal of Biological Control, 38 (4). pp. 494-503. ISSN 0971930X

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Abstract

Crotalaria ramosissima Roxb. is a Fabaceae family genus that prolifically grows as a weed in southern regions of India and is known for insect-repellent activity. The purpose of the current study is to assess the efficacy of anti-termite activity of the different solvents extracts and formulations of C. ramosissima against Odontotermes obesus which is prevalent in the south dry zone that destroys 10% of crops and wood on an average by feeding. The extracts showed anti-termite efficacy by No-choice bioassay method where the ethyl acetate extract showed highest termite mortality rates of 24.23±1.51%, 43.93±1.51%, 74.23±1.51% at 12, 24, 36h time intervals and methanol extract showed 16.66±1.51%, 30.29±4.00%, 54.54±2.62% at 12, 24, 36 h time intervals. The TLC analysis of ethyl acetate extract resulted in three bands which were separated through column chromatography and named Fraction-1, Fraction-2, and Fraction-3 and upon further investigation Fraction-3 showed maximum mortality rate of 81.81±2.62% at 36 hrs of time interval with a lethal concentration (LC-50) 248.44mg/ml. The GC-MS profiling of fraction-3 led to the identification of compounds viz. 1,2-benzenedicarboxylic acid, 1,2,4-oxadiazole, 3-(1,3-benzodioxol-5-yl)-5- [2-(4-methoxyphenyl)ethyl, phenmethylamine-2-acetoxy-n,n-bis[2-(benzoimid-2-yl)ethyl], and 9-octadecenoic acid (z)-2-hydroxyethyl ester and phenol, p-(1-ethyl-1-methylbutyl.These results may aid in the synthesis of novel effective bioactive compounds that needs to be explored for their toxicity against insects, as was enlightened in the current study.

Item Type:	Article
Uncontrolled Keywords:	Crotalaria ramosissima; GC-MS analysis; herbal-pesticide; termites
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Mr Eranna Library
Date Deposited:	29 Nov 2025 10:36
Last Modified:	16 Jul 2026 05:56
URI:	http://eprints.uni-mysore.ac.in/id/eprint/18101

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