Bienzymatic Spectrophotometric Method for Uric Acid Estimation in Human Serum and Urine

Roopa, R. A. and Mantelingu, K. and Guin, Mridula and Sridhar, B. T. (2022) Bienzymatic Spectrophotometric Method for Uric Acid Estimation in Human Serum and Urine. Journal of Analytical Chemistry, 77 (3). pp. 301-307. ISSN 1608-3199

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Abstract

In this study, a novel and efficient bienzymatic method for the quantification of uric acid in serum and urine samples was developed. This method is based on the bienzymatic reaction of uricase and peroxidase in the presence of substrates pyrocatechol and 3-methyl-2-benzothiazolinone hydrazone to produce a red colored product. Under optimized conditions, a linearity of uric acid assay was obtained in the ranges of 4 to 384 mu M and 2 to 256 mu M by kinetic method and fixed time method, respectively. The low limit of detection and limit of quantification were found to be 0.5 and 1.6 mu M, respectively. The developed assay was used to quantify uric acid in human serum and urine samples. The present method has good recovery range of 98.3-101.8% and accuracy range of 92.0-101.4%. Hence, the proposed method could be successfully adopted for the quantification of uric acid in clinical laboratories.

Item Type:	Article
Uncontrolled Keywords:	uricase; peroxidase; pyrocatechol; 3-methyl-2-benzothiazolinone hydrazone; serum; urine
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	06 Sep 2023 06:46
Last Modified:	06 Sep 2023 06:46
URI:	http://eprints.uni-mysore.ac.in/id/eprint/17735

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