Genotoxic and cytotoxic properties of zinc oxide nanoparticles phyto-fabricated from the obscure morning glory plant ipomoea obscura (L.) Ker Gawl

Murali, M. and Anandan, Satish and Ansari, Mohammad Azam and Alzohairy, Mohammad A. and Alomary, Mohammad N. and Asiri, Sarah Mousa Maadi and Almatroudi, Ahmad and Thriveni, M. C. and Singh, Sudarshana Brijesh and Gowtham, H. G. and Aiyaz, Mohammed and Srinivasa, Chandrashekar and Urooj, Asna and Amruthesh, K. N. (2021) Genotoxic and cytotoxic properties of zinc oxide nanoparticles phyto-fabricated from the obscure morning glory plant ipomoea obscura (L.) Ker Gawl. Molecules, 26 (4). pp. 1-16.

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Abstract

The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (similar to 24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL(-1). In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.

Item Type:	Article
Uncontrolled Keywords:	cytotoxicity; genotoxicity; cell cycle analysis by flow cytometry; ZnO-NPs; HT-29 cells; Allium cepa; Ipomoea obscura
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Mr Umendra uom
Date Deposited:	17 May 2022 09:44
Last Modified:	16 Jul 2022 06:58
URI:	http://eprints.uni-mysore.ac.in/id/eprint/17245

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