Bhat, M. K. and Prasad, B. N. and Veerabasappa Gowda, T. (1991) Purification and characterization of a neurotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom. Toxicon, 29 (11). pp. 1345-1349.
Full text not available from this repository. (Request a copy)Abstract
Snake venoms contain multimolecular forms of phospholipase A2 which are diverse with respect to their pharmacological properties. A neurotoxic PLA2 from Naja naja naja venom has been purified in two steps. (1) The whole venom was fractionated on CM-Sephadex C-25 column; 4.6% of the total PLA2 activity recovered was found in the NN-V fraction. (2) The NN-Vb-PLA2 fraction was purified to homogeneity by gel filtration of fraction NN-V on Sephadex G-50. It is a basic protein with a mol. wt between 10,500 and 11,000, and is more toxic than other basic PLA2s purified from Naja naja naja venom. The LD50 Of NN-Vb-PLA2 is 0.27 mg/kg body wt. It induced neurotoxic symptoms in experimental mice and is devoid of myotoxic, anticoagulant and edema-inducing activities.
| Item Type: | Article |
|---|---|
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 10 May 2021 05:35 |
| Last Modified: | 15 Jul 2022 06:51 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16403 |
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