Quantification of Sclerospora graminicola in tissues of Pennisetum glaucum using an enzyme‐linked immunosorbent assay

Kumar, V. U. and Meera, M. S. and Shishupala, S. and Shetty, H. S. (1993) Quantification of Sclerospora graminicola in tissues of Pennisetum glaucum using an enzyme‐linked immunosorbent assay. Planat Pathology, 42 (2). pp. 251-255.

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Abstract

Antibodies raised against whole sporangia of Sclerospora graminicola reacted specifically with sporangia and mycelium of this fungus. Agglutination studies localized the antigen on the cell-wall surfaces. Using this antibody, an indirect enzyme-linked immunosorbent assay (ELISA) with a biotin-avidin amplification system was developed for the quantification of S. graminicola in the host tissues of Pennisetum glaucum. This sensitive assay system, which could detect 3.1-5.5 mug dry weight of fungal biomass, was used to monitor the increase in fungal biomass during disease progression.

Item Type:	Article
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Users 23 not found.
Date Deposited:	05 May 2021 08:07
Last Modified:	13 Dec 2022 06:02
URI:	http://eprints.uni-mysore.ac.in/id/eprint/16322

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