Kulkarni, Venkatesh B. and Achar, Raghu Ram and Mahadevappa, Maheshwari and Dinesh, S. M. and Shubha, Priya Babu and Puttappa, Sharanappa and Swamy, Shivananju Nanjunda (2020) Hemostatically potent small molecular weight serine protease from Maclura spinosa (Roxb. ex Willd.) accelerates healing of subcutaneous dermal wounds in Swiss albino mice. Biologia, 75 (1). pp. 139-149.
Full text not available from this repository. (Request a copy)Abstract
Latex of Maclura spinosa has been used by Kollamalayali tribal community of Western Ghats for curdling of milk. In our previous study we have reported the presence of multiple proteases in the latex, among which the serine protease possesses potent role in hemostasis. With a view to further characterize these proteases the current study was taken up. Maclura spinosa latex contains a serine protease which is resolved using molecular size exclusion column chromatography and an anion exchanger resin in a consecutive manner. The specific activity of the enzyme Maclura spinosa latex protease (MSLP) was found to be 56.15 units/mg and recovery to be 2.68% with a fold purity of 0.41. Being a typical serine protease, MSLP is significantly inhibited by PMSF up to 72.97%. The optimum temperature and pH for the enzyme were found to be 50 degrees C and 8 respectively. Excision wound healing assay in Swiss albino mice using MSLP, showed accelerated wound closure up to 89.35 +/- 1.209% compared to 91.938 +/- 1.649% shown by positive control. Further PMSF treated MSLP sample did not show considerable wound healing which confirms exclusive involvement of serine proteases. Examination of biochemical markers viz, hydroxy proline content in healing tissue and catalase activity of fatty tissue also ascertain the potential of MSLP in wound healing. Histopathological studies of healing tissue provide confirmatory evidence with dense collagenation of tissue and fibroblast proliferation rendered by MSLP in the respective treated subjects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Maclura spinosa; Maclura spinosa latex protease; Serine protease; Hemostasis; Swiss albino mice; Wound healing |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Mr Umendra uom |
| Date Deposited: | 01 Feb 2021 10:11 |
| Last Modified: | 16 Nov 2022 11:17 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/15671 |
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