Anti-quorum sensing and biofilm inhibitory activity of Apium graveolens L. oleoresin

Nagar, Nupur and Aswathanarayan, Jamuna Bai and Vittal, Ravishankar Rai (2020) Anti-quorum sensing and biofilm inhibitory activity of Apium graveolens L. oleoresin. Journal of Food Science and Technology volume, 57 (7). pp. 2414-2422. ISSN 0975-8402

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Abstract

Apium graveolens L. (Apiaceae) is a dietary herb used as a spice, condiment and medicine. A. graveolens (Celery) has been studied for its antimicrobial property and for its application as flavours in food industry. The present study investigated the Apium graveolens oleoresin as an anti-quorum sensing and antibiofilm agent. The quorum sensing and biofilm inhibition study was carried out using biosensor strains Chromobacterium violaceum CV12472 and Pseudomonas aeruginosa PAO1. The MIC of celery oleoresin against C. violaceum CV12472 and P. aeruginosa PAO1 was 10 and 25% v/v, respectively. Inhibition of violacein and biofilm formation was tested at concentrations of oleoresins ranging from 1.56 and 50% v/v. The oleoresins showed a concentration dependent QS inhibitory activity and at sub-MIC of 6.25 and 12.5% v/v, the oleoresins significantly inhibited violacein production and biofilm formation (p < 0.05). Similarly, the celery oleoresin had significant QS modulatory effect on swimming, swarming and twitching motility in P. aeruginosa PAO1 at 12.5% v/v (p < 0.05). The major phytoconstituents present in celery oleoresin as analysed by GC-MS were eicosadiene, benzenemethanol and methyl ester which have not been previously reported. The findings suggest that celery has QS and biofilm inhibitory potential against gram negative pathogens and can find application as food intervention techniques.

Item Type:	Article
Subjects:	B Life Science > Microbiology
Divisions:	Department of > Microbiology
Depositing User:	Mr Umendra uom
Date Deposited:	05 Feb 2021 05:41
Last Modified:	05 Feb 2021 05:41
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15648

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