Bisdemethoxycurcumin promotes apoptosis in human platelets via activation of ERK signaling pathway

Paul, Manoj and Manikanta, K. and Hemshekhar, M. and Sundaram, Mahalingam S. and Naveen, S. and Ramesh, T. N. and Kemparaju, K. and Girish, K. S. (2020) Bisdemethoxycurcumin promotes apoptosis in human platelets via activation of ERK signaling pathway. Toxicology in Vitro, 63. ISSN 0887-2333

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Abstract

Curcumin, a major bioactive component of turmeric (Curcuma longa), is known for its multiple health benefits. Curcumin as such is a mixture of its analogs: bisdemethoxycurcumin (BDMC)-3%, and demethoxycurcumin (DMC)-17%. Although the effect of curcumin on platelets is documented, the effect of BDMC and DMC on platelets is less studied. Considering the indispensable role played by platelets in hemostasis, thrombosis, inflammation, and immunity, the present study evaluates the effect of curcumin, DMC and BDMC on platelet apoptosis. The components of curcumin were purified by silica-gel column chromatography. The purity and mass analysis of the purified curcuminoids was determined by RP-HPLC and LC-MS respectively. When analyzed for platelet apoptotic markers, only BDMC demonstrated increased incidence of platelet apoptotic markers including increase in intracellular Ca2+, decrease in Delta psi m, alteration in BCl-2 family proteins, the release of cytochrome c, caspase activation, and PS externalization via activation of ERK activation. ERK inhibitor PD98059 significantly alleviated BDMC induced decrease in Delta psi m, alteration in BCl-2, caspase-8 activation and PS externalization. Our results demonstrate that curcumin, DMC and BDMC differentially act on platelet in inducing apoptosis and the study highlights that the toxicity associated with curcumin therapy might be attributed to BDMC in the mammalian system.

Item Type:	Article
Uncontrolled Keywords:	Curcumin; BDMC platelet; Apoptosis; ERK signaling
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Mr Umendra uom
Date Deposited:	12 Feb 2021 06:06
Last Modified:	06 Dec 2022 09:53
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15626

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