Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi

Lakshmeesha, T. R. and Murali, M. and Ansari, Mohammad Azam and Udayashankar, Arakere C. and Alzohairy, Mohammad A. and Almatroudi, Ahmad and Alomary, Mohammad N. and Asiri, Sarah Mousa Maadi and Ashwini, B. S. and Kalagatur, Naveen Kumar and Nayak, Chandra S. and Niranjana, S. R. (2020) Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi. Saudi Journal of Biological Sciences, 27 (8). pp. 1923-1930. ISSN 2213-7106

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Abstract

Present study, report the biofabrication of zinc oxide nanoparticles from aqueous leaf extract of Melia azedarach (MaZnO-NPs) through solution combustion method and their novel application in preventing the growth of seed-borne fungal pathogens of soybean (Cladosporium cladosporioides and Fusarium oxysporum). The standard blotter method was employed to isolate fungi and was identified through molecular techniques. The characterization of MaZnO-NPs was carried out by UV-Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) equipped with Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The physicochemical characterization confirmed the particles were of high purity and nano size (30-40 nm) with a hexagonal shape. The synthesized MaZnO-NPs inhibited the growth of C. cladosporioides and F. oxysporum in a dose dependent manner. Biomass, ergosterol, lipid peroxidation, intracellular reactive oxygen species and membrane integrity determination upon MaZnO-NPs treatment offered significant activities there by confirming the mechanism of action against the test pathogens. In conclusion, due to the effectiveness of MaZnO-NPs in controlling the growth of C. cladosporioides and F. oxysporum, the synthesized MaZnO-NPs provides insight towards their potential application in agriculture and food industries. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Item Type:	Article
Uncontrolled Keywords:	Melia azedarach; Zinc oxide nanoparticles; Soybean; Seed-borne fungi; C. cladosporioides; F. oxysporum
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	Mr Umendra uom
Date Deposited:	25 Feb 2021 05:34
Last Modified:	25 Feb 2021 05:34
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15537

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