Neema, K. N. and Vivek, H. K. and Nafeesa Zohara and Divya Kumar and Shubha Priya Babu and Nagendra Prasad, M. N. and Nanjunda Swamy, S. (2020) Serine protease from Indian Cobra venom: its anticoagulant property and effect on human fibrinogen. Toxin Reviews, 41 (1). pp. 165-174. ISSN 1556-9551
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An unusual low molecular weight serine protease (NnP28) has been purified from Indian Cobra (Naja naja) venom from the western region of the Indian sub-continent. We reported the purification and characterization of low molecular NnP28, emphasizing its role on human fibrinogen and anticoagulant property. NnP28 was purified using gel filtration column chromatography followed by ion exchange chromatography. Protein gel electrophoresis revealed its molecular weight approximate to 28 kDa. The exact molecular mass of NnP28 was found to be 27.12 kDa by mass spectrometry, hydrolyzing casein specifically, inhibited by PMSF suggesting it has a serine protease. NnP28 prolonged the clotting time of re-calcified human citrated plasma and activated partial thromboplastin time (APTT) exhibiting anticoagulant property. NnP28 exhibited fibrinogenolytic activity. Thus, the present study demonstrates the presence of unusual low molecular weight serine protease, emphasizing its importance of region-specific Indian cobra species.
| Item Type: | Article |
|---|---|
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Mr Umendra uom |
| Date Deposited: | 30 Mar 2021 10:52 |
| Last Modified: | 30 Jun 2022 10:14 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/15470 |
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