Synergistic interaction of a protease and protease inhibitors from Russell's viper (Vipera russelli) venom

Jayanthi, G. P. and Veerabasappa Gowda, T. (1990) Synergistic interaction of a protease and protease inhibitors from Russell's viper (Vipera russelli) venom. ScienceDirect, 28 (1). pp. 65-74.

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Abstract

Synergistic interaction of a protease and protease inhibitors from Russell's viper (Vipera russelli) venom. Toxicon28, 65–74, 1990.—An acidic proteolytic enzyme, RVVX, was purified from Vipera russelli venom by successive chromatography on CM-Sephadex C-25, DEAE-cellulose and Sephadex G-100 columns. RVVX is a glycoprotein with a mol. wt of 79,000. It exhibited caseinolytic and factor X activating properties. Two trypsin inhibitors, TI-I and TI-II, were purified from V. russelli venom in a single step by CM-Sephadex C-25 column chromatography. The trypsin inhibitors interacted with the proteolytic enzyme RVVX. TI-I inhibited only the factor X activating property of RVVX while TI-II inhibited both, the caseinolytic and also factor X activating properties of RVVX. The edema inducing activity of RVVX increased markedly in the presence of non-edema inducing doses of TI-I and TI-II. RVVX, TI-I and TI-II were non-lethal in mice. The combination of RVVX and TI-II demonstrated enhanced toxicity.

Item Type:	Article
Uncontrolled Keywords:	Pharmacology & Pharmacy; Toxicology
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Dr Raju C
Date Deposited:	21 Jan 2021 09:41
Last Modified:	12 Jul 2022 07:53
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15435

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