Purification and characterization of a myotoxic phospholipase-a2 from indian cobra (Naja-Naja-Naja) venom

Bhat, M. K. and Gowda, T. V. (1989) Purification and characterization of a myotoxic phospholipase-a2 from indian cobra (Naja-Naja-Naja) venom. Toxicon, 27 (8). pp. 861-873.

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Abstract

Purification and characterization of a myotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom. Toxicon27, 861–873, 1989.—A major phospholipase A2 (NN-XIII-PLA2) which constitutes 20% of the whole Naja naja naja venom was purified to homogeneity on CM-Sephadex C-25 column chromatography. NN-XIII-PLA2 is a basic protein with a mol. wt of 11,200 by SDS-PAGE. This enzyme has low enzymatic activity but is more toxic to mice than the whole venom. The ld50 value (i.p.) of NN-XIII-PLA2 is 2.4 mg/kg body weight (whole venoms ld50 is 2.8 mg/kg body weight). It induces neurotoxic-like signs in experimental animals. It induces myotoxicity when injected i.m. into the thigh muscle of mice and edema when injected into the foot pads of mice. This enzyme has a fluorescence maxima between 310–316 nm which is typical of tyrosine residues.

Item Type:	Article
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Dr Raju C
Date Deposited:	21 Jan 2021 09:58
Last Modified:	09 May 2023 03:45
URI:	http://eprints.uni-mysore.ac.in/id/eprint/14259

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