Rapid quantitative assay of albendazole in bulk drug and pharmaceuticals by UHPLC

Swamy, N. and Basavaiah, K. and Penmatsa, V. K. and Vinay, K. B. and Prashanth, K. N. (2013) Rapid quantitative assay of albendazole in bulk drug and pharmaceuticals by UHPLC. Chemical Sciences Journal, 4 (1).

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Abstract

A simple, rapid, precise, isocratic and stability indicating ultra-high performance liquid chromatography (UHPLC) method was developed and validated for the determination of albendazole (ALB) in bulk drug and its dosage forms. The chromatographic separation of the drug was achieved on an Agilent Zorbax Extend C-18 (50 3 4.6 mm, 1.8 mm) UHPLC column with mobile phase consisting of a mixture of orthophosphoric acid buffer and acetonitrile (50:50 v/v). The eluted compound was detected at 295 nm with a UV detector. The newly developed method was validated according to the International Conference on Harmonisation (ICH) guidelines with respect to linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, robustness and ruggedness. The standard curve of mean peak area versus concentration showed an excellent linearity over a concentration range 0.24�60 mg mL21 ALB with regression coefficient (r) of 0.9998. The LOD (S/N 5 3) and LOQ signal-to-noise (S/N 5 10) were 0.01 and 0.04 mg mL21, respectively. ALB active substance was subjected to forced degradation to demonstrate the stability indicating power of the UHPLC method. Acidic, basic, oxidative, hydrolytic, thermal and photolytic degradation were used to assess the stability of the method. Peak purity index was checked using UV detection to demonstrate the specificity and stability indicating nature of the method.

Item Type: Article
Uncontrolled Keywords: Albendazole and UHPLC and Stability Indicating and Assay and Pharmaceuticals
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 07 Jan 2020 10:00
Last Modified: 07 Jan 2020 10:00
URI: http://eprints.uni-mysore.ac.in/id/eprint/10427

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