A simple and rapid nuclear staining method for Rhizoctonia solani Kuhn

Seema, M. and Punith, B. D. and Devaki, N. S. (2012) A simple and rapid nuclear staining method for Rhizoctonia solani Kuhn. Biotechnic & Histochemistry, 87 (3). pp. 169-171. ISSN 1473-7760

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Official URL: https://doi.org/10.3109/10520295.2011.577097

Abstract

AbstractWe developed a modified staining technique using acridine orange to stain the nuclei of Rhizoctonia solani. Acridine orange solution was prepared in acetic acid buffer, pH 7.2. Staining for 15 min was critical for observing the nuclei. All of the isolates were found to be multinucleated. The nuclei appeared bright green with light orange background. This method is simple, rapid and reproducible.

Item Type: Article
Subjects: B Life Science > Molecular Biology
Divisions: Yuvaraj college > Molecular biology
Depositing User: MUL SWAPNA user
Date Deposited: 22 Jul 2019 10:26
Last Modified: 22 Jul 2019 10:26
URI: http://eprints.uni-mysore.ac.in/id/eprint/5440

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