Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels

Suresh, C. and Dubey, A. K. and Kini, K. Ramachandra and Umesh Kumar, S. and Karanth, N. G. (1999) Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels. ELECTROPHORESIS, 20 (3). pp. 483-485.

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Official URL: http://dx.doi.org/10.1002/(SICI)1522-2683(19990301...

Abstract

A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. The reagent reacts with the reducing group of glucose released by glucoamylase from the substrate starch. Using the reaction, production of glucoamylase by Aspergillus niger was detected on 10% IEF gels within a pH range of 2.5-9.5. Since the method can detect raw and gelatinized starch activities of glucomylase associated with 1 mu g protein, it will be useful for enzyme engineering studies that involve screening of various mutations.

Item Type: Article
Uncontrolled Keywords: isoelectric focusing; glucoamylase; raw starch hydrolysis; direct detection
Subjects: Agriculture Biological Sciences > Botany
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Botany

Agriculture Biological Sciences > Biotechnology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biotechnology
Divisions: PG Campuses > Manasagangotri, Mysore > Biotechnology
PG Campuses > Manasagangotri, Mysore > Botany
Depositing User: Users 13 not found.
Date Deposited: 19 Apr 2013 13:33
Last Modified: 17 Aug 2013 05:55
URI: http://eprints.uni-mysore.ac.in/id/eprint/4958

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