Physico-chemical and biological studies on three-dimensional porous silk/spray-dried mesoporous bioactive glass scaffolds

Chandrasekaran, Arunseshan and Novajra, Giorgia and Carmagnola, Irene and Gentile, Piergiorgio and Fiorilli, Sonia and Miola, Marta and Manjunatha, B. and Dakshanamoorthy, Arivuoli and Ciardelli, Gianluca and Vitale-Brovarone, Chiara (2016) Physico-chemical and biological studies on three-dimensional porous silk/spray-dried mesoporous bioactive glass scaffolds. Ceramics International, 42 (12). 13761 - 13772.

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The incorporation of a bioactive inorganic phase in polymeric scaffolds is a good strategy for the improvement of the bioactivity and the mechanical properties, which represent crucial features in the field of bone tissue engineering. In this study, spray-dried mesoporous bioactive glass particles (SD-MBG), belonging to the binary system of SiO2-CaO (80:20mol), were used to prepare composite scaffolds by freeze-drying technique, using a silk fibroin matrix. The physico-chemical and biological properties of the scaffolds were extensively studied. The scaffolds showed a highly interconnected porosity with a mean pore size in the range of 150µm for both pure silk and silk/SD-MBG scaffolds. The elastic moduli of the silk and silk/SD-MBG scaffolds were 1.1±0.2MPa and 6.9±1.0MPa and compressive strength were 0.5±0.05MPa and 0.9±0.2MPa, respectively, showing a noticeable increase of the mechanical properties of the composite scaffolds compared to the silk ones. The contact angle value decreased from 105.3° to 71.2° with the incorporation of SD-MBG particles. Moreover, the SD-MBG incorporation countered the lack of bioactivity of the silk scaffolds inducing the precipitation of hydroxyapatite layer on their surface already after 1 day of incubation in simulated body fluid. The composite scaffolds showed good biocompatibility and a good alkaline phosphatase activity toward human mesenchymal stromal cells, showing the ability for their use as three-dimensional constructs for bone tissue engineering.

Item Type: Article
Uncontrolled Keywords: Silk fibroin, Mesoporous bioactive glass, Composite scaffolds, Bone tissue engineering
Subjects: B Life Science > Sericulture
Divisions: Department of > Sericulture Science
Depositing User: manjula User
Date Deposited: 20 Jun 2019 06:27
Last Modified: 14 Nov 2019 10:05

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