Optimisation of extraction of bioactive compounds from Feronia limonia (wood apple) fruit using response surface methodology (RSM)

Ilaiyaraja, N. and Likhith, Kr. and Babu, G. R. S. and Khanum, F. (2015) Optimisation of extraction of bioactive compounds from Feronia limonia (wood apple) fruit using response surface methodology (RSM). FOOD CHEMISTRY, 173. pp. 348-354. ISSN 1873-7072

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Official URL: https://doi.org/10.1016/j.foodchem.2014.10.035

Abstract

In this study, the extraction conditions for the maximum recovery of polyphenols with high antioxidant activity were optimised by response surface methodology (RSM) in Feronia limonia fruit. The independent variables were viz, concentration of ethanol (X-1: 30-70%), incubation temperature (X-2: 37-60%) and solvent-to-solid ratio (X-3: 20-40%). ANOVA results showed that concentration of ethanol and temperature affected the total polyphenol content (TPC, Y-1), DPPH (Y-2) and ABTS (Y-3) radical scavenging activities significantly (p < 0.05) whereas solvent-to-solid ratio was found to be insignificant. A second-order polynomial model satisfactorily fitted the experimental data with the R-2 values of 0.966, 0.946 and 0.955, respectively for the responses Y-1, Y-2 and Y-3 (p < 0.0001), implying a good agreement between the predicted and experimental values. The optimal conditions for the highest yield of TPC (7.21 +/- 1.4 g GAE/g) with >80% radical scavenging activities were derived at X-1 = 62.7%, X-1 = 49.7 degrees C and X-3 = 39.4 mL/g. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type: Article
Subjects: B Life Science > Food Science and Nutrition
Divisions: Department of > Food Science and Nutrition
Depositing User: Shrirekha N
Date Deposited: 11 Jun 2019 09:38
Last Modified: 11 Jun 2019 09:38
URI: http://eprints.uni-mysore.ac.in/id/eprint/2746

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