Isolation and characterization of an endogenous inhibitor of phospholipase A(2) from Indian cobra (Naja naja naja) venom

Rudrammaji, L. M. S. and Veerabasappa Gowda, T. (1998) Isolation and characterization of an endogenous inhibitor of phospholipase A(2) from Indian cobra (Naja naja naja) venom. Toxicon, 36 (4). pp. 639-644.

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Official URL: https://doi.org/10.1016/S0041-0101(97)00096-2

Abstract

A PLA(2)-inhibitor has been purified from Indian cobra (Naja naja naja) venom by the combination of ion-exchange and gel-filtration chromatography. The inhibitor, NN-I-3 was a peptide with mol.wt 6500 and has a fluorescence emission maxima cn 340 nm. NN-I-3 specifically inhibited the enzyme activity of the three acidic PLA? from the same venom. The inhibition of NN-I-2d-PLA(2) and NN-I-2e-PLA(2) by NN-I-3 was of mixed type and NN-I-2c-PLA(2) was of uncompetitive type, Neither the inhibitor nor the individual mixtures of acidic PLA(2) with the inhibitor (1:1 w/w or 1:2 mol:mol) were lethal to mice when injected intraperitoneally in doses up to 10 mgkg(-l) body weight. (C) 1998 Elsevier Science Ltd. All rights reserved.

Item Type: Article
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Users 23 not found.
Date Deposited: 07 Jun 2021 03:48
Last Modified: 12 Jul 2022 06:48
URI: http://eprints.uni-mysore.ac.in/id/eprint/16724

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