Serine protease from Indian Cobra venom: its anticoagulant property and effect on human fibrinogen

Neema, K. N. and Hamse Kameshwar, Vivek and Nafeesa, Zohara and Kumar, Divya and Babu Shubha, Priya and Nagendra Prasad, M. N. and Swamy, Shivananju Nanjunda (2020) Serine protease from Indian Cobra venom: its anticoagulant property and effect on human fibrinogen. Toxin Reviews. ISSN 1556-9551

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Official URL: https://www.tandfonline.com/doi/full/10.1080/15569...

Abstract

An unusual low molecular weight serine protease (NnP28) has been purified from Indian Cobra (Naja naja) venom from the western region of the Indian sub-continent. We reported the purification and characterization of low molecular NnP28, emphasizing its role on human fibrinogen and anticoagulant property. NnP28 was purified using gel filtration column chromatography followed by ion exchange chromatography. Protein gel electrophoresis revealed its molecular weight approximate to 28 kDa. The exact molecular mass of NnP28 was found to be 27.12 kDa by mass spectrometry, hydrolyzing casein specifically, inhibited by PMSF suggesting it has a serine protease. NnP28 prolonged the clotting time of re-calcified human citrated plasma and activated partial thromboplastin time (APTT) exhibiting anticoagulant property. NnP28 exhibited fibrinogenolytic activity. Thus, the present study demonstrates the presence of unusual low molecular weight serine protease, emphasizing its importance of region-specific Indian cobra species.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Mr Umendra uom
Date Deposited: 30 Mar 2021 10:52
Last Modified: 30 Mar 2021 10:52
URI: http://eprints.uni-mysore.ac.in/id/eprint/15470

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