Development and validation of two stability- indicating Uv-spectrophotometric methods for the determination of repaglinide in bulk and dosage forms

Xavier, C. M. and Basavaiah, K. and Ramesh, P. J. and Vinay, K. B. (2013) Development and validation of two stability- indicating Uv-spectrophotometric methods for the determination of repaglinide in bulk and dosage forms. International Journal of ChemTech Research, 5 (1). pp. 72-79.

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Abstract

Two stability indicating UV-spectrophotometric methods were developed and validated for the determination of repaglinide with its degradation products. Assay of repaglinide in 0.1M NaOH and 0.1M HCl were achieved at 216nm and 243nm respectively. The correlation was in the range of 1.0 – 25 µg mL-1 (r = 0.9999) for 0.1M NaOH and 2.0 – 40 µg mL-1 (r = 0.9999) for 0.1M HCl with molar absorptivity values of 2.02 × 104 L mol-1 cm-1 and Sandell sensitivity of 0.0224 µg cm-2 for 0.1M NaOHand 1.17 × 104 L mol-1 cm-1 and Sandell sensitivity of 0.0386 µg cm-2 for 0.1M HCl. The detection limit and quantification limit are calculated to be (0.13, 0.39 µg mL-1) and (0.18 and 0.54 µg mL-1) for 0.1M NaOH and 0.1M HCl respectively. The method was validated for accuracy, precision, robustness, selectivity and applied for marketed formulations with less than 2% relative error and standard deviation.

Item Type:	Article
Uncontrolled Keywords:	analytical parameters, article, calibration, drug degradation, drug determination, drug dosage form, drug sensitivity, drug stability, eurepa 1, eurepa 2, high performance liquid chromatography, limit of detection, limit of quantitation, oxidation, process development, repaglinide, robustness, ruggedness, ultraviolet spectrophotometry, unclassified drug, validation process
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	30 Oct 2019 09:39
Last Modified:	30 Oct 2019 09:39
URI:	http://eprints.uni-mysore.ac.in/id/eprint/9669

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