Inhibition of hyaluronidase by N-acetyl cysteine and glutathione: Role of thiol group in hyaluronan protection

Sunitha, K. and Suresh, P. and Santhosh, M. S. and Hemshekhar, M. and Thushara, R. M. and Marathe, G. K. and Thirunavukkarasu, C. and Kemparaju, K. and Kumar, M. S. and Girish, K. S. (2013) Inhibition of hyaluronidase by N-acetyl cysteine and glutathione: Role of thiol group in hyaluronan protection. International Journal of Biological Macromolecules, 55. pp. 39-46. ISSN 0141-8130

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Hyaluronidase inhibitors have immense applications in pathophysiological conditions associated with hyaluronan-hyaluronidase system. The present study demonstrates the inhibitory efficacy of clinically accepted antioxidant N-acetyl cysteine (NAC) against hyaluronidase of serum, testis, and snake and bee venoms. The experimental and molecular dynamic simulation data suggest the non-competitive inhibition and involvement of thiol groups of both NAC and glutathione in exertion of inhibition. The bioavailability, less-toxic and antioxidant nature of NAC and glutathione could become valuable in the management of pathologies triggered by extracellular matrix degradation and to increase the endurance of hyaluronan based biomaterials/supplements, which are highly exciting aspects.

Item Type: Article
Uncontrolled Keywords: article, controlled study, unclassified drug, Animals, antioxidant, enzyme inhibition, glutathione, Glutathione, hyaluronidase, Hyaluronoglucosaminidase, Kinetics, snake venom, hyaluronic acid, molecular docking, Molecular Docking Simulation, Protein Binding, enzyme blood level, testis, degradation, Molecular Dynamics Simulation, bioavailability, extracellular matrix, Molecular Conformation, molecular dynamics, pathophysiology, acetylcysteine, Acetylcysteine, enzyme inhibitor, Enzyme Inhibitors, tissue level, bee venom, biomaterial, hyaluronidase antibody, thiol group
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 31 Oct 2019 10:06
Last Modified: 31 Oct 2019 10:06

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