An efficient technique to obtain conidial inoculum of Peronosclerospora sorghi causing downy mildew of sorghum

Singh, H. and Garampalli, R. H. (2013) An efficient technique to obtain conidial inoculum of Peronosclerospora sorghi causing downy mildew of sorghum. Archives of Phytopathology and Plant Protection, 46 (6). pp. 656-662. ISSN 1477-2906

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Official URL: http://doi.org/10.1080/03235408.2012.749699

Abstract

An effective and simple technique to obtain conidial inoculum of Peronosclerospora sorghi has been devised, which confers many advantages over the conventional method. Systemically infected sorghum leaves were packed into folds of paper-towel. The end of folded paper-towel containing cut-ends of leaves was dipped in 3 cm deep water in a container. The container was exposed to moist field conditions at night and sporulated leaves were harvested with chilled distilled water in the morning to obtain the inoculum. Advantages of the technique include lower cost and effort, rapid harvesting and daily production of inoculum for up to seven consecutive days from same leaves. Though conventional method of plating leaf pieces in Petri plates proves superior in terms of the amount of sporulation, paper-towel method has the edge under working conditions.

Item Type: Article
Uncontrolled Keywords: Peronosclerospora sorghi, Peronosporaceae
Subjects: B Life Science > Botany
Divisions: Department of > Botany
Depositing User: Arshiya Kousar
Date Deposited: 12 Nov 2019 09:35
Last Modified: 12 Nov 2019 09:35
URI: http://eprints.uni-mysore.ac.in/id/eprint/9635

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