In vitro antioxidant activity, lipoxygenase, cyclooxygenase-2 inhibition and dna protection properties of Memecylon species

Sekhar, S. and Sampath Kumara, K. K. and Niranjana, S. R. and Prakash, H. S. (2013) In vitro antioxidant activity, lipoxygenase, cyclooxygenase-2 inhibition and dna protection properties of Memecylon species. International Journal of Pharmacy and Pharmaceutical Sciences, 5 (SUPPL.). pp. 257-262.

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Abstract

Traditionally several species of Memecylon are used to cure skin problems. However, scientific validation of the plant as a modern medicine is lacking. Therefore, in the present study methanolic extracts of three species of Memecylon i.e., M. umbellatum, M. talbotianum and M. malabaricum were assessed for their antioxidant, anti-microbial, DNA protection capacities, 5-lipoxygenase and human cyclooxygenase (COX-2) inhibition. Methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) ABTS radical scavenging capacities for extracts in dose range of 50 μg/mL - 10 mg/mL were measured. 15-Lipoxygenase inhibition for these extracts at 10 - 200 μg/mL dose was studied. Human cyclooxygenase-2 was recorded for doses 10 and 50 μg/mL. DNA-nicking assay at 10 μg was recorded. Cell cytotoxicity was recorded by colorimetric MTT assay using doxorubicin as control for extracts (50 - 500 μg/mL). Results: The IC50 values for scavenging the DPPH radical ranged from 0.11 to 0.17 mg/mL and those for the ABTS radical cation from 2.1 to 3.7 mg/mL for the extracts. M. malabaricum leaf extract exhibited highest lipoxygenase inhibition capacity with an IC50 value of 29.87 μg/mL. It also inhibited human COX-2 (80.6 % at 50 μg/mL). Methanolic leaf extracts could prevent DNA nicking by hydroxyl radicals, produced in the Fenton reaction. M. malabaricum leaf extracts inhibited growth of Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli. Conclusion: This study forms initial screening of these plants with further studies directed towards identification of bioactive molecules as potential lead candidates.

Item Type: Article
Uncontrolled Keywords: article, human, controlled study, nonhuman, 1, 1 diphenyl 2 picrylhydrazyl, antioxidant activity, arachidonate 15 lipoxygenase, plant extract, unclassified drug, medicinal plant, cyclooxygenase 2, enzyme inhibition, Escherichia coli, Staphylococcus aureus, antimicrobial activity, IC 50, ultraviolet spectrophotometry, phenol, Klebsiella pneumoniae, lipoxygenase, cell mediated cytotoxicity, memecylon extract, memecylon malabaricum, memecylon talbotianum, memecylon umbellatum
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 09 Dec 2019 10:12
Last Modified: 17 Dec 2019 11:15
URI: http://eprints.uni-mysore.ac.in/id/eprint/9600

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