Vinay, K. B. and Revenasiddappa, H. D. and Abdulrahman, S. A. M. (2013) Sensitive spectrophotometric determination of tramadol hydrochloride in pharmaceuticals using folin-ciocalteu's reagent. Turkish Journal of Pharmaceutical Sciences, 10 (1). pp. 57-68.
![[img]](http://eprints.uni-mysore.ac.in/style/images/fileicons/text.png) |
Text (Full Text)
Che_2013_Revanasiddappa_09.pdf - Published Version Restricted to Registered users only Download (8MB) | Request a copy |
Abstract
A simple, rapid and sensitive method is described for the determination of tramadol hydrochloride (TMH) in pure form or in tablets. The method is based on the reduction of Folin-Ciocalteu (F-C) reagent by TMH in alkaline medium, leading to the formation of a blue coloured chromogen, which absorbs maximally at 760 nm. Under the optimized experimental conditions, the colour is stable for over 12 h and Beer's law is obeyed in the concentration range of 2.5-50 μ/mL. The regression coefficient (r) is calculated to be 0.9998 with molar absorptivity value of 6.3 � 103 L/mol/cm and Sandell sensitivity of 0.0478 μ/cm per 0.001 absorbance unit. The limits of detection (LOD) and quantification (LOQ) are also reported. Within-day variation determined on three different concentrations showed accuracies ranging from 0.70 to 1.53% and the RSD was determined to be < 2%. Day to day variation presented accuracies ranging from 1.12 to 1.53% with an RSD < 2%. The proposed method was successfully applied to the determination of TMH in tablets with good recoveries.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | accuracy, alkalinity, article, chromogenic substrate, concentration process, controlled study, drug determination, drug structure, folin ciocalteu, limit of detection, limit of quantitation, reaction optimization, reaction time, reagent, sensitivity analysis, spectrophotometer, spectrophotometry, tablet formulation, tramadol, unclassified drug |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Arshiya Kousar Library Assistant |
| Date Deposited: | 14 Nov 2019 06:17 |
| Last Modified: | 22 Jun 2022 09:36 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/9599 |
Actions (login required)
 |
View Item |
