Vani, N. and Mohan, B. and Nagendrappa, G. (2013) A new high-performance thin-layer chromatographic method for determination of diazepam in spiked blood samples. Journal of Planar Chromatography - Modern TLC, 26 (4). pp. 343-348.
Full text not available from this repository. (Request a copy)Abstract
A high-performance thin-layer chromatographic method combined with densitometric measurement and UV scanning was used to optimize a new mobile phase consisting of hexane and ethyl acetate (7:3 v/v) for the estimation of diazepam in spiked human blood. Efficacy and ease of extraction with three solvents, acetonitrile, diethyl ether, and chloroform, each at three different pH conditions were studied. Validation parameters for diazepam were found to be linear in the range of 0.5-20 μg/spot with r = 0.998, limit of detection (LOD), 0.2 μg, limit of quantification (LOQ), 0.5 μg. Usefulness of the new mobile phase for the separation of six common benzodiazepines, namely, nitrazepam, clonazepam, lorazepam, chlordiazepoxide, alprazolam, and clozapine from diazepam, was proved through their retention factor and UV pattern.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | accuracy, acetic acid ethyl ester, acetonitrile, alprazolam, article, benzodiazepine derivative, Blood, chlordiazepoxide, chloroform, Chromatographic analysis, Chromatographic methods, Chromatography, clonazepam, clozapine, Densitometric measurement, densitometry, diazepam, Diazepam, drug blood level, drug determination, drug isolation, ether, Extraction, hexane, high performance thin layer chromatography, HPTLC-densitometry, human, limit of detection, Limit of quantifications, limit of quantitation, lorazepam, Mobile phase, nitrazepam, Organic solvents, quantitative analysis, reproducibility, ultraviolet spectrophotometry, UV pattern, Validation parameters, validity |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Arshiya Kousar Library Assistant |
| Date Deposited: | 18 Nov 2019 07:07 |
| Last Modified: | 18 Nov 2019 07:07 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/9583 |
Actions (login required)
 |
View Item |
