Synergistically acting PLA2: Peptide hemorrhagic complex from Daboia russelii venom

Venkatesh, M. and Veerabasappa Gowda, T. (2013) Synergistically acting PLA2: Peptide hemorrhagic complex from Daboia russelii venom. Toxicon, 73. pp. 111-120. ISSN 1879-3150

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Snake venoms are complex mixture of enzymatic and non-enzymatic proteins. Non-covalent protein-protein interaction leads to protein complexes, which bring about enhanced pharmacological injuries by their synergistic action. Here we report identification and characterization of a new Daboia russelii hemorrhagic complex I (DR-HC-I) containing phospholipase A2 (PLA2) and non-enzymatic peptide. DR-HC-I was isolated from the venom of D. russelii by CM-Shepadex-C25 and gel permeation chromatography. Individual components were purified and identified by RP-HPL chromatography, mass spectrometry and N-terminal amino acid sequencing. DR-HC-I complex was lethal to mice with the LD50 dose of 0.7mg/kg body weight with hemorrhagic and neurotoxic properties. DR-HC-I complex consists of non-hemorrhagic PLA2 and neurotoxic non-enzymatic peptide. The non-enzymatic peptide quenched the intrinsic fluorescence of PLA2 in a dose dependent manner, signifying the synergistic interaction between two proteins. PLA2 and peptide toxin in a 5:2M ratio induced skin hemorrhage in mice with MHD 20μg. However, addition of ANS (1-Anilino-8-naphthalene sulfonate) to DR-HC-I complex inhibited skin hemorrhagic effect and also synergic interaction. But there was no impact on PLA2 due to this synergistic interaction, and indirect hemolytic or plasma re-calcification activity. However, the synergistic interaction of PLA2 and non-enzymatic peptide contributes to the enhanced venom-induced hemorrhage and toxicity of Daboia russellii venom.

Item Type: Article
Uncontrolled Keywords: 1-anilino-8-naphthalenesulfonate, 8 anilino 1 naphthalenesulfonic acid, amino acid sequence, Amino Acid Sequence, Anilino Naphthalenesulfonates, animal, animal experiment, animal model, Animals, article, calcification, chemically induced, Chromatography, controlled study, Daboia russelii hemorrhagic complex I, Daboia russellii, DNA, DNA sequence, enzymology, fluorescence, Fluorescence, Gel, gel chromatography, hemolysis, Hemorrhage, high performance liquid chromatography, High Pressure Liquid, LD 50, Lethal Dose 50, lethality, male, mass spectrometry, Mass Spectrometry, metabolism, Mice, molecular genetics, Molecular Sequence Data, mouse, multiprotein complex, Multiprotein Complexes, neurotoxicity, nonhuman, nucleotide sequence, peptide, Peptides, phospholipase A2, Phospholipases A2, priority journal, protein determination, protein function, protein isolation, protein protein interaction, protein purification, Russell's Viper, Sequence Analysis, skin bleeding, Skin Diseases, snake venom, toxicity, unclassified drug, viper venom, Viper Venoms
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 26 Nov 2019 10:52
Last Modified: 15 Jul 2022 06:55

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