Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Olanzapine in Human Urine

Anil Kumar, U. R. and Bhasakra, L. B. and Basavaiah, K. (2012) Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Olanzapine in Human Urine. ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING, 37 (5). pp. 1381-1387.

Full text not available from this repository. (Request a copy)
Official URL: http://dx.doi.org/10.1007/s13369-012-0249-7

Abstract

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine olanzapine (OLZ) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C-18 column (Symmetry 3.5 mu m, 50 x 4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of ammonium acetate (pH 7.8)-acetonitrile (10:90, v/v). The method was linear over a concentration range of 1-100 ngml(-1). The lower limit of quantitation was 1 ngml(-1). The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was < 11.5 %. The accuracy determined at three concentrations (8.0, 50.0 and 85.0 ngml(-1) OLZ) was within +/- 1.21 % in terms of relative errors.

Item Type: Article
Uncontrolled Keywords: Olanzapine; Quantification; Mass spectrometry
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Swamy D
Date Deposited: 16 May 2013 11:15
Last Modified: 28 Oct 2015 05:31
URI: http://eprints.uni-mysore.ac.in/id/eprint/9368

Actions (login required)

View Item View Item