In vitro propagation from inflorescence explants of anisochilus carnosus-an ethno-medicinal herb

Reshi, N. A. and Sudarshana, M. S and Nandini, B. P. (2014) In vitro propagation from inflorescence explants of anisochilus carnosus-an ethno-medicinal herb. International Journal of Pharmaceutical Sciences and Research, 5 (6). pp. 2423-2427. ISSN 0975-8232

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Abstract

A protocol was devised for callus induction and in vitro micro propagation of Anisochilus carnosus. Leaf, nodal and immature inflorescence explants were cultured for callusing on solid MS medium with best callusing response achieved at higher concentrations of BAP (12mg/l) alone and in combination with 2,4-D (3mg/l) and IAA (5mg/l). Bud regeneration from inflorescence callus giving maximum (9.67±0.58) shoots per bud was achieved at 12mg/l BAP+5mg/l IAA. Elongated shoots were transferred to half strength MS liquid medium for in vitro rhizogenesis, with best rooting achieved in 2mg/l IBA giving maximum number of 13.00±1.00 roots per shoot. The rooted plantlets were hardened by transferring to polycups containing sterile soil and vermicompost in the ratio of 1:1. After 15 days of hardening, established plantlets were transferred to field with maximum survivability.

Item Type:	Article
Uncontrolled Keywords:	Callusing and Bud Induction and Multiple Shoots and Acclimatisation and Anisochilus Carnosus
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Users 19 not found.
Date Deposited:	21 Oct 2019 05:21
Last Modified:	21 Oct 2019 05:21
URI:	http://eprints.uni-mysore.ac.in/id/eprint/9351

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